Description
DDDDK Antibody [1D1B12] (biotin) | PM-7657-biotin | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Tag
Homology: N/A
Immunogen: Biotin-DDDDK antibody was raised against a synthetic peptide containing DYKDDDDK.
Research Area: Other
Tested Application: E, WB
Application: Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ/ml.
Specificiy: Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Biotin-DDDDK antibody is affinity chromatography purified via peptide column.
Clonality: Monoclonal
Clone: 1D1B12
Isotype: IgG
Conjugate: Biotin
Physical State: Liquid
Buffer: Biotin-DDDDK antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Biotin-DDDDK antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Biotin-DDDDK, DDDDK, FLAG, FLAGtag, flag-tag
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.