DDI2 Antibody (Center) [APR05691G]

DDI2 Antibody (Center) [APR05691G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This DDI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-196 amino acids from the Central region of human DDI2.

Cellular Localisation: Cytoplasm, cytosol. Chromosome

Molecular Weight: 44523

Clone: Polyclonal

Gene Name: DDI2 (HGNC:24578)

Gene ID: 84301

Function: Aspartic protease that mediates the cleavage of NFE2L1/NRF1 at 'Leu-104', thereby promoting release of NFE2L1/NRF1 from the endoplasmic reticulum membrane (PubMed:27676298, PubMed:27528193) . Ubiquitination of NFE2L1/NRF1 is a prerequisite for cleavage, suggesting that DDI2 specifically recognizes and binds ubiquitinated NFE2L1/NRF1 (PubMed:27528193) . Seems to act as a proteasomal shuttle which links the proteasome and replication fork proteins like RTF2 (Probable) . Required, with DDI1, for cellular survival following replication stress. Together or redudantly with DDI1, removes RTF2 from stalled forks to allow cell cycle progression after replication stress and maintains genome integrity (PubMed:29290612) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:500 WB--1:1000

Synonyms: Protein DDI1 homolog 2, DDI2