DDX39B monoclonal Antibody | MB62993

DDX39B monoclonal Antibody | MB62993 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Monkey, Mouse, Rat, Dog

Application: WB

Application Range: WB 1:500

Background: Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly with [PubMed:23299939] reporting a stimulatory effect. [UniProtKB/Swiss-Prot Function]

Storage & Stability: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Specificity: DEAD-box helicase 39B

Molecular Weight: 48.8 kDa (Predicted)

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: BAT1; D6S81E; UAP56

Immunogen: Human recombinant protein fragment corresponding to amino acids 319-428 of human DDX39B (NP_004631) produced in E.coli.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Pathway: N/A