DEPTOR Antibody | 7787

DEPTOR Antibody | 7787 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.
The immunogen is located within amino acids 220 - 270 of DEPTOR.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P

Application: DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 45 kDa
Observed: 42 kDa

Validation: N/A

Isoform: N/A

Purification: DEPTOR antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DEPTOR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DEPTOR Antibody: DEP.6, DEPDC6, DEP domain-containing mTOR-interacting protein, DEP domain-containing protein 6

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DEPTOR is an mTOR (mammalian target of rapamycin) -interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1) . Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1) . In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2) .