DetectX® Triiodothyronine (T3) ELISA Kit

The DetectX^® Triiodothyronine (T₃) Enzyme Immunoassay kit is designed to quantitatively measure T₃ present in urine, extracted serum and plasma, extracted dried fecal samples, and tissue culture media samples. This kit measures total T₃ in extracted serum and plasma and in extracted fecal samples.

A T₃ stock solution is provided to generate standard curves for the assay. Standards or diluted samples are pipetted into a coated microtiter plate. A T₃-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T₃ to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T₃-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm.

• Sample - Urine, Media, and Extracted Serum, Plasma and Fecal Extracts
• Samples/Kit - 39 or 231 in Duplicate
• Sensitivity - Measure < 40 pg/mL

DetectX® Triiodothyronine (T3) ELISA Kit DataSheet