DFHBI-1T | B2799

DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.

DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Displays peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. DFHBI-1T has been found to have lower background fluorescence than DFHBI (Cat. No. B2797) and exhibits an overall increase in brightness in living cells. DFHBI-1T is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Spinach™, Spinch2™, and Broccoli™ RNA tags.

B2799 | DFHBI-1T DataSheet

Alternate Name/Synonyms: DFHBI1T,(Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one

Appearance: Yellow solid

Formulation:

CAS Number: 1539318-36-9

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₃H₉F₅N₂O₂

Molecular Weight: 320.21

Cell-Permeable?: TRUE

Purity: ≥98% by HPLC

Solubilities: >15 mg/ml Ethanol, >30 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A GFP fluorophore mimic for imaging RNA in living cells

MDL Number: MFCD31628972

PubChem CID: 101889712

SMILES: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F

InChi: InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-

InChi Key: AWYCLBWNRONMQC-WMZJFQQLSA-N