DFHBI | B2797

DFHBI is a cell-permeable non-fluorescent small molecule derived from the GFP chromophore. Upon binding to the “Spinach™” aptamer, DFHBI is converted to a highly fluorescent state that can be detected at the emission wavelength of 501 nm. DFHBI displays negligible toxicity in living cells. DFHBI can be used to label any genetically encoded Spinach™ family of RNA tags that include Spinach™, Spinach2™, and Broccoli™. Live-cell imaging of tagged RNA can be performed using a standard fluorescence microscopy. Further, DFHBI can also selectively detect tagged RNA in total RNA gel electrophoresis.

DFHBI is a cell-permeable non-fluorescent small molecule derived from the GFP chromophore. Upon binding to the “Spinach™” aptamer, DFHBI is converted to a highly fluorescent state that can be detected at the emission wavelength of 501 nm. DFHBI displays negligible toxicity in living cells. DFHBI can be used to label any genetically encoded Spinach™ family of RNA tags that include Spinach™, Spinach2™, and Broccoli™. Live-cell imaging of tagged RNA can be performed using a standard fluorescence microscopy. Further, DFHBI can also selectively detect tagged RNA in total RNA gel electrophoresis.

B2797 | DFHBI DataSheet

Alternate Name/Synonyms: (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl- 4H-Imidazol-4-one,3’5’-difluoro-4-hydroxybenzylidene

Appearance: Yellow solid

Formulation:

CAS Number: 1241390-29-3

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₂H₁₀F₂N₂O₂

Molecular Weight: 252.22

Cell-Permeable?: TRUE

Purity: ≥95% by HPLC

Solubilities: ~20 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A cell-permeable, non-toxic fluorogen

MDL Number:

PubChem CID: 70808995

SMILES: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1C

InChi: InChI=1S/C12H10F2N2O2/c1-6-15-10(12(18)16(6)2)5-7-3-8(13)11(17)9(14)4-7/h3-5,17H,1-2H3/b10-5-

InChi Key: ZDDIJYXDUBFLID-YHYXMXQVSA-N