DGKZ Antibody (C-term) [APR32540G]

DGKZ Antibody (C-term) [APR32540G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ.

Cellular Localisation: Nucleus. Cytoplasm, cytosol. Cell membrane. Cell projection, lamellipodium

Molecular Weight: 103981

Clone: Polyclonal

Gene Name: DGKZ (HGNC:2857)

Gene ID: 8525

Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095) . Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095) . Also plays an important role in the biosynthesis of complex lipids (Probable) . Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:9159104, PubMed:19744926, PubMed:22108654) . Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129) . The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity) . By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668) . Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity) .

Summary: Tissue Location: Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, IF

Dilution: WB--1:1000 IHC-P--1:100~500 IF--1:10~50

Synonyms: Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta, DGKZ, DAGK6