Diacylglycerol kinase iota (DGKI) Antibody (C-term) [APR15734G]

Diacylglycerol kinase iota (DGKI) Antibody (C-term) [APR15734G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This Diacylglycerol kinase iota (DGKI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 890-920 amino acids from the C-terminal region of human Diacylglycerol kinase iota (DGKI) .

Cellular Localisation: Cell projection, axon {ECO:0000250|UniProtKB:F1MAB7}. Cell projection, dendrite {ECO:0000250|UniProtKB:F1MAB7}. Cell junction, synapse, presynapse {ECO:0000250|UniProtKB:F1MAB7}. Cell junction, synapse, postsynapse {ECO:0000250|UniProtKB:F1MAB7}. Cell junction, synapse, postsynaptic density {ECO:0000250|UniProtKB:F1MAB7}. Cell junction, synapse, synaptic cell membrane {ECO:0000250|UniProtKB:F1MAB7}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:F1MAB7}. Cytoplasm, cytosol. Nucleus Note=Excluded from inhibitory synapses (By similarity) . Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018) . Both in the detergent soluble and particulate fractions (By similarity) . {ECO:0000250|UniProtKB:F1MAB7, ECO:0000269|PubMed:9830018}

Molecular Weight: 116997

Clone: Polyclonal

Gene Name: DGKI (HGNC:2855)

Gene ID: 9162

Function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9830018, PubMed:23949095) . Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable) . Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position (PubMed:9830018) . By controlling the diacylglycerol/DAG- mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression (By similarity) .

Summary: Tissue Location: Specifically expressed in brain and retina (PubMed:9830018) . In brain, highly expressed in hippocampus, caudate nucleus, occipital pole, cerebral cortex, and cerebellum (PubMed:9830018) . Also detected in kidney (PubMed:15894621)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Diacylglycerol kinase iota, DAG kinase iota, Diglyceride kinase iota, DGK-iota, DGKI