DIAPH1 Antibody (N-term) [APR15737G]

DIAPH1 Antibody (N-term) [APR15737G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DIAPH1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 18-52 amino acids from the N-terminal region of human DIAPH1.

Cellular Localisation: Cell membrane {ECO:0000250|UniProtKB:O08808}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:O08808} Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm {ECO:0000250|UniProtKB:O08808}. Nucleus {ECO:0000250|UniProtKB:O08808} Note=Membrane ruffles, especially at the tip of ruffles, of motile cells. {ECO:0000250|UniProtKB:O08808}

Molecular Weight: 141347

Clone: Polyclonal

Gene Name: DIAPH1

Gene ID: 1729

Function: Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (By similarity) . Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization (By similarity) . Required for cytokinesis, and transcriptional activation of the serum response factor (By similarity) . DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity) . Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity) . Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane (By similarity) . In hear cells, it may play a role in the regulation of actin polymerization in hair cells (PubMed:20937854, PubMed:21834987, PubMed:26912466) . The MEMO1-RHOA- DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex (PubMed:20937854, PubMed:21834987) . It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity (PubMed:20937854, PubMed:21834987) . In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (PubMed:20937854, PubMed:21834987) . Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape (PubMed:20937854, PubMed:21834987) . Plays a role in brain development (PubMed:24781755) . Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (By similarity) .

Summary: Tissue Location: Expressed in brain, heart, placenta, lung, kidney, pancreas, liver, skeletal muscle and cochlea. Expressed in platelets (PubMed:26912466) .

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:500-1:1000

Synonyms: Protein diaphanous homolog 1, Diaphanous-related formin-1, DRF1, DIAPH1, DIAP1