DIRAS3 Antibody | 8139

DIRAS3 Antibody | 8139 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
The immunogen is located within the last 50 amino acids of DIRAS3.

Research Area: Cell Cycle, Cancer, Autophagy

Tested Application: E, WB

Application: DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: DIRAS3 antibody is human specific.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 25 kDa
Observed: 24 kDa

Validation: N/A

Isoform: N/A

Purification: DIRAS3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DIRAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1) . Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1) . DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR) , upregulating ATG4, and colocalizing with LC3 in autophagosomes (2) . DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3) .