DIS3 Antibody (Center) [APR03425G]

DIS3 Antibody (Center) [APR03425G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DIS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-323 amino acids from the Central region of human DIS3.

Cellular Localisation: Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus Note=Predominantly located in the nucleus (PubMed:20531386) . According to PubMed:12429849, found in the nucleolus (PubMed:12429849) . According to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 (PubMed:20531386)

Molecular Weight: 109003

Clone: Polyclonal

Gene Name: DIS3

Gene ID: 22894

Function: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Summary: Tissue Location: Widely expressed.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, WB

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Exosome complex exonuclease RRP44, 3113-, 3126-, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, DIS3, KIAA1008, RRP44