DIS3L2 Antibody | 8347

DIS3L2 Antibody | 8347 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: DIS3L2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DIS3L2.
The immunogen is located within amino acids 720 - 770 of DIS3L2.

Research Area: Homeostasis

Tested Application: E, WB

Application: DIS3L2 antibody can be used for detection of DIS3L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: DIS3L2 antibody is human, mouse and rat reactive. At least three isoforms of DIS3L2 are known to exist; this antibody will only detect the longest isoform. DIS3L2 is predicted to not cross-react with DIS3 or DIS3L.

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 97 kDa
Observed: 94 kDa

Validation: N/A

Isoform: N/A

Purification: DIS3L2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DIS3L2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DIS3L2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DIS3 like 3'-5' exoribonuclease 2, FAM6A, PRLMNS

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The exosome is involved in a multitude of cellular RNA processing and degradation events (1) . DIS3, also known as exosome complex exonuclease RRP44, is a ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species (2) . DIS3L2 is a paralog of DIS3, but unlike DIS3 is located in the cytoplasm (3) . DIS3L2 defines a novel eukaryotic RNA degradation pathway and preferentially targets uridylated RNA (4) . DIS3L2 mutations have also been associated with sporadic Wilms tumors (3) .