DISP2 Antibody | 4893

DISP2 Antibody | 4893 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: DISP2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human DISP2.
The immunogen is located within amino acids 110 - 160 of DISP2.

Research Area: Stem Cell

Tested Application: E, IHC-P

Application: DISP2 antibody can be used for detection of DISP2 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: DISP2 antibody is human specific. At least two isoforms of Disp2 are known to exist. DISP2 antibody is predicted to not cross-react with Disp1 or Disp3.

Positive Control 1: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: DISP2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DISP2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DISP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: DISP2 Antibody: DISPB, HsT16908, DISPB, KIAA1742, Protein dispatched homolog 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DISP2 Antibody: DISP2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the DISP proteins have been implicated in these pathways. Recently, it has been shown that DISP2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased DISP2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by DISP2 mutants.