DKK1 Antibody | 45-049

DKK1 Antibody | 45-049 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow

Immunogen: The immunogen for this antibody is: CDHHQASNSSRLHT

Research Area: Signal Transduction

Tested Application: E, WB, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 38kDa band observed in Human Bone Marrow and Spleen lysates (calculated MW of 28.7kDa according to NP_036374.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Biol Chem. 1999 Jul 2;274 (27) :19465-72; PMID: 10383463) . Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Spleen for this particular batch.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: Human Bone Marrow Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis) , dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1, dickkopf related protein-1

User Note: N/A

BACKGROUND: N/A