DMPO | DMPO nitrone adduct (clone N1664A) | AS11 1632

DMPO | DMPO nitrone adduct (clone N1664A) | AS11 1632 | Gentaur UK, US & Europe Distribution

Immunogen: 5, 5-dimethyl-2- (8-octanoic acid) -1-pyrrolone-N-oxide conjugated to ovalbumin

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG1

Purity: Total IgG. Protein G purified.

Format: Liquid

Tested Application: ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)

Related Products: collection of antibodies to proteins involved in oxidative stressSecondary antibodies

Recommended Dilutions: 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)

Molecular weight: 90 kDa

Confirmed Reactivity: DMPO (species independent)

Predicted Reactivity: DMPO (species independent)

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct

Background: DMPO (5, 5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Reconstitution: N/A

Storage: Store at -20°C for one year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Oxidative stress

Research Area: Proteomics