DNA Binding Protein-7 (DBP-7), human recombinant | 7603

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete -barrel capped at the opening by a C-terminal -helix; they bind to the minor groove of a DNA duplex via the triple-stranded -sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

7603 | DNA Binding Protein-7 DBP-7 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: DBP, DNA Binding Protein-7 (DBP-7), human recombinant

Alternates names: apm-1, Adipolean , 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28

Taglines: Promotes annealing of complementary DNA strands

NCBI Gene ID #: 246253

NCBI Gene Symbol: ADIPOQ

Gene Source: Human

Accession #: NP_653345

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED for this effect is < 1-3 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 16.6 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives

Reconstitution Instructions: Reconstitute in HO to a concentration of <0.3 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A