DNA Library Prep Kit for IIlumina Sequencing - K1475

K1475 | DNA Library Prep Kit for IIlumina Sequencing DataSheet

’s DNA Library Prep Kit for IIlumina Sequencing is used to efficiently convert (2 ng-500 ng) of DNA fragments into fully compatible libraries for Illumina Next Generation Sequencing (NGS) platforms. The sample preparation step involves the preparation of a DNA library by fragmentation, end repair, adenylation, ligation with adapter sequences and amplification. The DNA library is then loaded onto the chips for amplification and sequencing. The presence of adapter sequences allows the hybridization of the libraries to the sequencing chips and provides a universal priming site for sequencing primers. ’s DNA Library Prep Kit contains index adapters needed for running multiple samples on a single Illumina flow cell as well as the magnetic beads for purification, which is required for the reaction cleanup after ligation and PCR steps. The entire procedure can be completed within 2 hours.

Alternate Name :

Tag Line : To prepare DNA samples for Illumina Sequencing

Summary : DNA library is prepared for Illumina sequencing by end repair, adenylation, ligation with adapter sequences and amplification

Detection Method : DNA library is prepared for Illumina Sequencing by end repair, adenylation, ligation with adapter sequences and PCR amplification

Sample Type : Purified fragmented DNA (recommended size range of 200-500 bp) or PCR amplicons

Species Reactivity :

Applications : Library preparation for Whole Genome Sequencing, ChIP-Seq, Amplicon Sequencing and cDNA Sequencing

Features and Benefits : Wide range of input amounts (2-500 ng) and sample types, including amplicons and fragmented DNA,High quality Illumina compatible libraries,Minimal hands on time and short 2 hour library preparation workflow