DNA Ligase I (E139) polyclonal Antibody | BS2528

DNA Ligase I (E139) polyclonal Antibody | BS2528 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DNA Ligase I (E139) polyclonal Antibody detects endogenous levels of DNA Ligase I protein.

Molecular Weight: ~ 102 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DNA ligase 1; DNA ligase I; DNLI1; LIG 1; lig1; Ligase I DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 1;

Immunogen: Synthetic peptide, corresponding to amino acids 110-160 of Human DNA Ligase I.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: