DNA Polymerase I, Large (Klenow) Fragment | P1597

The Large, Klenow Fragment is a proteolytic product of E. coli DNA Polymerase I, which retains 5’→ 3’ polymerase activity and 3’→ 5’ exonuclease activity. It is useful for generating blunt ends by removing the 3’ overhangs and filling the 5’ overhangs. It can be also used to generate probes and second strand cDNA.

P1597 | DNA Polymerase I, Large (Klenow) Fragment DataSheet

Biomolecule/Target :

Synonyms: DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA Polymerase I, Klenow,DNA Polymerase I, (Klenow) Fragment,DNA Polymerase I, Large (Klenow) Fragment,2.7.7.7

Alternates names: DNA Polymerase I,DNA Polymerase I, Large Fragment,Klenow Fragment,DNA Polymerase I, Klenow,DNA Polymerase I, (Klenow) Fragment,DNA Polymerase I, Large (Klenow) Fragment,2.7.7.7

Taglines: A proteolytic product of E. coli DNA Polymerase I

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: E.Coli

Accession #:

Recombinant: YES

Source: E. coli

Purity by SDS-PAGE:

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 20,000 U/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.

Molecular Weight: 67.9 kDa

Concentration:

Appearance: Liquid protein

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: Klenow Fragment (amino acids 324 - 928)

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans