DNASE1L3 Antibody (N-term) [APR31169G]

DNASE1L3 Antibody (N-term) [APR31169G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: Rat

Specificity: This DNASE1L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human DNASE1L3.

Cellular Localisation: Nucleus. Endoplasmic reticulum. Secreted Note=Translocates from the endoplasmic reticulum to the nucleus during apoptosis (PubMed:23229555) . Contradictory reports exist about the subcellular localization under normal physiological conditions. Under conditions of cell death, may diffuse and/or be actively transported to the nucleus. {ECO:0000269|PubMed:23229555, ECO:0000305}

Molecular Weight: 35504

Clone: Polyclonal

Gene Name: DNASE1L3 (HGNC:2959)

Gene ID: 1776

Function: Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity) . Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:9070308, PubMed:9714828, PubMed:14646506, PubMed:10807908, PubMed:27293190) . Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463) . The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity) . Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190) . Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity) . NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity) . Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity) .

Summary: Tissue Location: Liver and spleen.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Deoxyribonuclease gamma, DNase gamma, 3121-, DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, DNase I-like 3, Liver and spleen DNase, LS-DNase, LSD, DNASE1L3, DHP2, DNAS1L3