DNMT3A Antibody (CenterR478) [APR31340G]

DNMT3A Antibody (CenterR478) [APR31340G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human DNMT3A.

Cellular Localisation: Nucleus. Chromosome Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin {ECO:0000250|UniProtKB:O88508}

Molecular Weight: 101858

Clone: Polyclonal

Gene Name: DNMT3A

Gene ID: 1788

Function: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:12138111, PubMed:16357870, PubMed:30478443) . DNA methylation is coordinated with methylation of histones (PubMed:12138111, PubMed:16357870, PubMed:30478443) . It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:12138111, PubMed:16357870, PubMed:30478443) . May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity) . Plays a role in paternal and maternal imprinting (By similarity) . Required for methylation of most imprinted loci in germ cells (By similarity) . Acts as a transcriptional corepressor for ZBTB18 (By similarity) . Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity) . Can actively repress transcription through the recruitment of HDAC activity (By similarity) . Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity) .

Summary: Tissue Location: Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: DNA (cytosine-5) -methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A