DOCK8 Antibody | 7847

DOCK8 Antibody | 7847 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8.
The immunogen is located within the last 50 amino acids of DOCK8.

Research Area: Immunology

Tested Application: E, WB, IHC-P

Application: DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 166, 220, 231 kDa
Observed: 220, 230 kDa

Validation: N/A

Isoform: N/A

Purification: DOCK8 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DOCK8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DOCK8 Antibody: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1) . DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2) . Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3) .