Dog ALB (Albumin) ELISA Kit | ELK1351

Dog ALB (Albumin) ELISA Kit | ELK1351 | Gentaur UK, US & Europe Distribution

Alternative Names: Serum albumin

Species: Dog

Assay Type: Competitive Inhibition

Sensitivity: 0.45 μg/mL

Standard: 100 μg/mL

Detection Range: 1.57-100 μg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Albumin (ALB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Albumin (ALB) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Albumin (ALB) in the samples is then determined by comparing the OD of the samples to the standard curve.