Dog MIP3b (Macrophage Inflammatory Protein 3 Beta) ELISA Kit | ELK5937

Dog MIP3b (Macrophage Inflammatory Protein 3 Beta) ELISA Kit | ELK5937 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL19; CKb11; ELC; MIP3-B; SCYA19; Chemokine C-C-Motif Ligand 19; Beta Chemokine Exodus-3; CK Beta-11; EBI1-Ligand Chemokine; Small Inducible Cytokine Subfamily A19

Species: Dog

Assay Type: Sandwich

Sensitivity: 5.6 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Beta (MIP3b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Beta (MIP3b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Beta (MIP3b) in the samples is then determined by comparing the OD of the samples to the standard curve.