Dog SELE (Selectin, Endothelium) ELISA Kit | ELK5973

Dog SELE (Selectin, Endothelium) ELISA Kit | ELK5973 | Gentaur UK, US & Europe Distribution

Alternative Names: CD62E; CD62-E; E-Selectin; ELAM; ELAM1; ESEL; SEL-E; E-LAM; E-LAM1; E-SEL; LECAM2; Endothelial Leukocyte Adhesion Molecule 1; CD62 Antigen-Like Family Member E

Species: Dog

Assay Type: Sandwich

Sensitivity: 2.84 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Selectin, Endothelium (SELE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Selectin, Endothelium (SELE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Selectin, Endothelium (SELE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Selectin, Endothelium (SELE) in the samples is then determined by comparing the OD of the samples to the standard curve.