Donkey anti-Sheep IgG (H&L), ALP conjugated | AS09 628

Donkey anti-Sheep IgG (H&L), ALP conjugated | AS09 628 | Gentaur UK, US & Europe Distribution

Immunogen: Purified sheep IgG, whole molecule

Host: Donkey

Conjugation: ALP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified donkey IgG.

Format: Liquid

Tested Application: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

Related Products: N/A

Recommended Dilutions: 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Molecular weight: N/A

Confirmed Reactivity: Sheep IgG heavy and light chains (H&L)

Predicted Reactivity: Sheep IgG Heavy and Light chains (H&L)

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background: Donkey anti-sheep IgG is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to all donkey immunoglobulins in immunological assays.

Reconstitution: N/A

Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: ALP (alkaline phosphatase)

Research Area: Immunoassays