Donkey anti-Sheep IgG (H&L), DyLight® 488 conjugated, min, cross-reactivity to human or rabbit IgG | AS09 636

Donkey anti-Sheep IgG (H&L), DyLight® 488 conjugated, min, cross-reactivity to human or rabbit IgG | AS09 636 | Gentaur UK, US & Europe Distribution

Immunogen: Purified Sheep IgG, whole molecule

Host: Donkey

Conjugation: DyLight® 488

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified donkey IgG.

Format: Lyophilized

Tested Application: N/A

Related Products: other DyLight® anti-Sheep secondary antibodies

Recommended Dilutions: 1 : 20-1 : 2000 for most applications

Molecular weight: N/A

Confirmed Reactivity: N/A

Predicted Reactivity: N/A

Not reactive in: N/A

Additional Information: BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Background: Donkey anti-Sheep IgG (H&L), DyLight®488 Conjugated, min. cross-reactivity to human or rabbit IgG is a secondary antibody conjugated to DyLight® 488, which binds to Sheep IgG (H&L) in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Sheep IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: DyLight® 488

Research Area: Immunoassays