Donkey anti-Sheep IgG (H&L), HRP conjugated | AS09 604

Donkey anti-Sheep IgG (H&L), HRP conjugated | AS09 604 | Gentaur UK, US & Europe Distribution

Immunogen: Purified sheep IgG, whole molecule

Host: Donkey

Conjugation: HRP

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified donkey IgG.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Related Products: AS09 611 | Donkey anti-sheep IgG (H&L) biotin conjugatedCollection of secondary antibodies

Recommended Dilutions: 1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)

Molecular weight: N/A

Confirmed Reactivity: Sheep IgG heavy chains and light chains on all sheep immunoglobulins

Predicted Reactivity: N/A

Not reactive in: non-immunoglobulin sheep serum proteins based in immunoelectrophoresis

Additional Information: N/A

Background: Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.

Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Centrifuge to remove any particulates, Prepare fresh working dilutions daily

Storage: Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

TAIR Nnumbre: N/A

Category: HRP (horse radish peroxidase)

Research Area: Immunoassays