Dopamine ELISA kit | 55R-IB89547

Dopamine ELISA kit | 55R-IB89547 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: Hormones & Steroids

Short Description: ELISA kit for the detection of Dopamine in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C.

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end-user

Assay Information: Dopamine is extracted by using a cisdiolspecific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.