DPP4/CD26, Human Recombinant | P1572

DPP4, dipeptidyl peptidase-4 is a complex enzyme expressed on the surface of most cell types and is a serine exopeptidase that cleaves x-proline dipeptides from the N-terminus of polypeptides. DPP4 protein is associated with intracellular signal transduction, apoptosis and plays an important role in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.

P1572 | DPP4/CD26, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: ADCP-2, ADABP, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD_antigen: CD26

Alternates names: ADCP-2, ADABP, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, TP103, CD_antigen: CD26

Taglines: Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation.

NCBI Gene ID #: 1803

NCBI Gene Symbol: DPP4

Gene Source: Human

Accession #: P27487

Recombinant: TRUE

Source: Baculovirus

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method): > 200 unit/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit produces 1.0 umole of p-Nitroaniline from Gly-Pro- p-Nitroaniline per minute at pH 8.0 at 37C.

Molecular Weight: 85.4 kDa (737aa) (C-terminal His-tag)

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM EDTA, 10% glycerol

Reconstitution Instructions:

Amino acid sequence: aa 39-766

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans