DPP4 protein | 30R-1195

DPP4 protein | 30R-1195 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Cell Cycle & Cell Death

Short Description: Purified recombinant Human DPP4 protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 39-766 amino acids: ADP-R KTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP-SGRLVPRGSHHHHHH

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: Hi-5 cells

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 0.5 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, containing 100mM NaCl, 1mM EDTA, and 10% glycerol

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Approximately > 50 Unit/mg. One unit will hydrolyze 1 uM of p-nitroaniline per minute at pH 8.0 and 37 DEG C using 1mM of Gly-Pro p-nitroanilde as a substrate.

Usage Recommendations: N/A

Assay Information: 1. Prepare a 80ul reaction mix in a suitable container: The final concentrations are 1mM EDTA, 20mM Tris pH8.0, 0.1M NaCl. 2. Add 10 ul of 10mM substrate (Gly-Pro p-nitroanilide) to each well. 3. Add 10ul of recombinant DPP-4 diluent to each well. 4. Incubate the 96 well plate at 37 DEG C for 10 min. 5. Read the optical density at 405 nm.