DUSP1 / MKP1 Antibody | 18-871

DUSP1 / MKP1 Antibody | 18-871 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: A synthetic peptide of human DUSP1 / MKP1

Research Area: Signal Transduction

Tested Application: WB, IF

Application: WB: 1:500 - 1:2000
IF: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: HepG2

Positive Control 2: BT-474

Positive Control 3: Mouse brain

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 49kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: DUSP1, dual specificity phosphatase 1, HVH1, CL100, MKP-1, PTPN10, serine/threonine specific protein phosphatase, MKP1, VH1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.