DUSP10, human recombinant | 7814

DuSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DuSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DuSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DuSP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7814 | DUSP10 human recombinant DataSheet

Biomolecule/Target: DUSP10

Synonyms: Dual specificity protein phosphatase 10, MKP5

Alternates names: Dual specificity protein phosphatase 10, MKP5

Taglines: Negatively regulates members of the MAPK superfamily

NCBI Gene ID #: Mm.252481

NCBI Gene Symbol: EGF

Gene Source: Human

Accession #: P01132

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 1600 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH 7.5 using 10 mM of substrate.

Results: Specific activity is > 1600 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 40.4 kDa (359 aa, 149-482 aa + His Tag)

Concentration: 0.25 mg/ml

Appearance: Liquid

Physical form description: 0.25 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2 M NaCl and 2 mM DTT

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV