DUSP21, human recombinant | 7816

DuSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases (DuSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DuSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7816 | DUSP21 human recombinant DataSheet

Biomolecule/Target: DUSP21

Synonyms: Dual specificity protein phosphatase 21

Alternates names: Dual specificity protein phosphatase 21

Taglines: Catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues.

NCBI Gene ID #: Mm.252481

NCBI Gene Symbol: EGF

Gene Source: Human

Accession #: P01132

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 1800 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH7.5 using 10 mM of substrate.

Results: Specific activity is > 1800 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.1 kDa (214 aa, 1-190 aa + His Tag)

Concentration: 0.25 mg/ml

Appearance: Liquid

Physical form description: 0.25 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM