DUSP23, human recombinant | 7817

DuSP23, also known as low molecular mass dual specificity phosphatase 3(LDP-3), belongs to the protein-tyrosine phosphatase family. This protein is a protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. This protein able to enhance activation of JNK and p38(MAPK14). Recombinant human DuSP23 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7817 | DUSP23 human recombinant DataSheet

Biomolecule/Target: DUSP23

Synonyms: Dual specificity protein phosphatase 23, DUSP25, LDP-3, MOSP, VHZ

Alternates names: Dual specificity protein phosphatase 23, DUSP25, LDP-3, MOSP, VHZ

Taglines: Catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues.

NCBI Gene ID #: Rn. 6075

NCBI Gene Symbol: EGF

Gene Source: Human

Accession #: P07522

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 320 unit/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37ºC, pH7.5 using 10 mM of substrate.

Results: Specific activity is > 320 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.8 kDa (170 aa, 1-150 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100 mM NaCl and 2 mM DTT

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGVQPPNFSW VLPGRLAGLA LPRLPAHYQF LLDLGVRHLV SLTERGPPHS DSCPGLTLHR LRIPDFCPPA PDQIDRFVQI VDEANARGEA VGVHCALGFG RTGTMLACYL VKERGLAAGD AIAEIRRLRP GSIETYEQEK AVFQFYQRTK