DUT Polyclonal Antibody | E-AB-12372

DUT Polyclonal Antibody | E-AB-12372 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: Deoxyuridine 5' triphosphate nucleotidohydrolase, nuclear isoform, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, Deoxyuridine triphosphatase, dut, DUT, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPase, FLJ20622

Application: WB, IHC, ELISA

Reactivity: Human, Rat

Host: Rabbit

Isotype: IgG

Reserch Areas: Epigenetics and Nuclear Signaling

Background: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Concentration: 0.2 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic peptide of human DUT

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification Method: Affinity purification

Dilution: WB 1:200-1:1000, IHC 1:10-1:50

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 27 kDa

Molecular Weight(Observed): N/A