DUX4 Antibody (Center) [APR30817G]

DUX4 Antibody (Center) [APR30817G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DUX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-275 amino acids from the Central region of human DUX4.

Cellular Localisation: [Isoform 1]: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108, ECO:0000269|PubMed:15709750, ECO:0000269|PubMed:17984056, ECO:0000269|PubMed:21060811, ECO:0000269|PubMed:26951377, ECO:0000269|PubMed:27378237, ECO:0000269|PubMed:28459457, ECO:0000269|PubMed:29618456} Note=Actively transported through the nuclear pore complex (NPC)

Molecular Weight: 44940

Clone: Polyclonal

Gene Name: DUX4 (HGNC:50800)

Gene ID: 100288687

Function: [Isoform 1]: Transcription factor that is selectively and transiently expressed in cleavage-stage embryos (PubMed:28459457) . Binds to double-stranded DNA elements with the consensus sequence 5'- TAATCTAATCA-3' (PubMed:28459457, PubMed:28459454, PubMed:29572508, PubMed:30540931, PubMed:30315230) . Binds to chromatin containing histone H3 acetylated at 'Lys-27' (H3K27ac) and promotes deacetylation of H3K27ac. In parallel, binds to chromatin that lacks histone H3 acetylation at 'Lys-27' (H3K27ac) and recruits EP300 and CREBBP to promote acetylation of histone H3 at 'Lys-27' at new sites (PubMed:26951377) . Involved in transcriptional regulation of numerous genes, primarily as transcriptional activator, but mediates also repression of a set of target genes (PubMed:17984056, PubMed:27378237, PubMed:26951377, PubMed:28459457, PubMed:28459454, PubMed:29618456, PubMed:30540931, PubMed:29572508) . Promotes expression of ZSCAN4 and KDM4E, two proteins with essential roles during early embryogenesis (PubMed:27378237, PubMed:26951377, PubMed:28459457, PubMed:29618456) . Heterologous expression in cultured embryonic stem cells mediates also transcription of HERVL retrotransposons and transcripts derived from ACRO1 and HSATII satellite repeats (PubMed:28459457) . May activate expression of PITX1 (PubMed:17984056) . May regulate microRNA (miRNA) expression (PubMed:24145033) . Inappropriate expression can inhibit myogenesis and promote apoptosis (PubMed:26951377, PubMed:28935672, PubMed:29618456) .

Summary: Tissue Location: Isoform 1: Does not seem to be expressed in normal muscle, but is detected in muscle of individuals with FSHD, and also in testis (at protein level) (PubMed:21060811, PubMed:17984056) . Isoform 1: Does not seem to be expressed in normal muscle, but in muscle of individuals with FSHD, where it may be toxic to cells (PubMed:21060811, PubMed:17984056) . Isoform 2: Detected in skeletal muscle, fibroblasts and testis from healthy individuals (PubMed:21060811)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC

Dilution: FC--1:25 WB--1:2000

Synonyms: Double homeobox protein 4 {ECO:0000312|HGNC:HGNC:50800}, Double homeobox protein 10 {ECO:0000312|EMBL:AAK915091}, DUX4 (HGNC:50800), DUX10