DYKDDDDK (binds to Sigma FLAG®, clone FG4R) | AS15 2871

DYKDDDDK (binds to Sigma FLAG®, clone FG4R) | AS15 2871 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated DYKDDDDK (FLAG) synthetic peptide

Host: Mouse

Conjugation: N/A

Clonality: Monoclonal

Isotype: IgG1

Purity: Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format: Liquid

Tested Application: Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)

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Recommended Dilutions: 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user

Molecular weight: N/A

Confirmed Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.

Reconstitution: N/A

Storage: Store at -20°C for up to 2 years.

TAIR Nnumbre: N/A

Category: DYKDDDDK (binds to SigmaFLAG®)

Research Area: Tag Antibodies