E. coli Recombinant Carbonic anhydrase | P1049

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3 + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

P1049 | E. coli Recombinant Carbonic anhydrase DataSheet

Biomolecule/Target: can

Synonyms: Carbonate dehydratase, CAN, yadF

Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a

Taglines: Catalyzes the reversible hydration of carbon dioxide in various biological processes.

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2

Gene Source: E. coli

Accession #: P01563

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg

Results: >1,000 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.4 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A