E. coli Recombinant IDNK | P1050

D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1050 | E. coli Recombinant IDNK DataSheet

Biomolecule/Target: idnK

Synonyms: ECK4261, gntV, JW4225

Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a

Taglines: Protein involved in the pathway L-idonate degradation, which is part of Carbohydrate acid metabolism.

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2

Gene Source: E. coli

Accession #: P01563

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.

Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg

Results: > 80 units/mg

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.

Molecular Weight: 19.4 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A