E. Coli Recombinant SlyD | 6366

SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.

6366 | E. Coli Recombinant SlyD DataSheet

Biomolecule/Target: N/A

Synonyms: E. Coli Recombinant SlyD

Alternates names: FKBP-type peptidyl-prolyl cis-trans isomerase.

Taglines: Exhibits several activities including that of a peptidyl-prolyl isomerase

NCBI Gene ID #: Rn. 6075

NCBI Gene Symbol: EGF

Gene Source: E. Coli

Accession #: P07522

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is >220 nmoles/min/mg.

Results: >220 nmoles/min/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 µmole of Suc-AAFP-pNA per minute at 25°C in Tris HCl pH 8.0 using chymotrypsin.

Molecular Weight: 21 kDa (196 aa, 1-196 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl (pH 7.5).

Reconstitution Instructions: N/A

Amino acid sequence: MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH