E2F1 Antibody | 18-476

E2F1 Antibody | 18-476 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: A synthetic peptide of human E2F1

Research Area: Apoptosis, Cancer, Cell Cycle, Signal Transduction, Transcription

Tested Application: WB, IF

Application: WB: 1:500 - 1:1000
IF: 1:20 - 1:100

Specificiy: N/A

Positive Control 1: A-431

Positive Control 2: BxPC-3

Positive Control 3: U-87MG

Positive Control 4: NIH/3T3

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 70kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: E2F1, E2F-1, RBBP3, RBP3, RBAP1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP) , a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.