EAAT1 / GLAST1 Antibody | 43-235

EAAT1 / GLAST1 Antibody | 43-235 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Rat

Immunogen: The immunogen for this antibody is: C-RSFKVPIQANET

Research Area: Signal Transduction

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3) . Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Specificiy: This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1) .

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 60kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: SLC1A3, solute carrier family 1 (glial high affinity glutamate transporter) , member 3, EA6, EAAT1, FLJ25094, GLAST, GLAST1, GLAST-1, OTTHUMP00000116002, excitatory amino acid transporter 1, sodium-dependent glutamate/aspartate transporter 1, solute carrier family 1 member 3

User Note: N/A

BACKGROUND: N/A