Competent Cells

ECOS™ 101 (DH5α) | FYE607-10VL/FYE607-80VL/FYE608-10VL/FYE608-80VL/FYE609-10VL/FYE609-80VL

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Description

ECOS™ 101 (DH5α) | FYE607-10VL/FYE607-80VL/FYE608-10VL/FYE608-80VL/FYE609-10VL/FYE609-80VL | Gentaur UK, US & Europe Distribution

DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

Features

Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

Applications
  • Cloning and subcloning
  • Scale-up application
  • Blue/white screening

Technical / Specifications

Store at -70°C

Genotype

endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk–, mk+) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ– F–
 
Specifications
 

# FYE678-10VL (10 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE678-80VL (80 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE607-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE607-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE608-10VL (10 preps)

Efficiency >3 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE608-80VL (80 preps)

Efficiency >3 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE609-10VL (10 preps)

Efficiency >1 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE609-80VL (80 preps)

Efficiency >1 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE607-96WL (96 preps)

Efficiency >1 x 108 cfu/μg
50 μl/well 96 wells

 

# FYE608-96WL (96 preps)

Efficiency >3 x 108 cfu/μg
50 μl/well 96 wells

 

# FYE609-96WL (96 preps)

Efficiency >1 x 109 cfu/μg
50 μl/well
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1 Review

  • 4
    genomic library

    Posted by Charles on 10th Sep 2022

    I use this ECOS X for applications which require very high transformation efficiencies like as for my genomic library construction with efficiency.

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