Competent Cells

ECOS™ Blue (XL1- Blue) | FYE107-10VL/FYE107-80VL/FYE108-10VL/FYE108-80VL/FYE109-10VL/FYE109-80VL

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SKU:
1-FYE107-10VL/1-FYE107-80VL/1-FYE108-10VL/1-FYE108-80VL/1-FYE109-10VL/1-FYE109-80VL-GEN
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NULL168.00 - NULL955.00

Description

ECOS™ Blue (XL1- Blue) | FYE107-10VL/FYE107-80VL/FYE108-10VL/FYE108-80VL/FYE109-10VL/FYE109-80VL | Gentaur UK, US & Europe Distribution

XL1-Blue is the most popular strain for blue/white screening. It is also an excellent host strain for routine cloning application using plasmid or lambda vectors. XL1-Blue cells are endonuclease (endA) deficient, which greatly improve the quality of mini-prep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned DNA by the EcoK endonuclease system. The lacIqZΔM15 gene on the F´ episome allows blue-white color screening.
 
Features
  • A strain modified to be ideal for blue/white screening
  • Tetracycline resistant

Applications

  • Routine cloning application
  • Subcloning
  • cDNA library
  • Blue/white screening

Store at -70°C

Genotype

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’proABlacIqZDM15 Tn10 (TetR)]
 
Specifications
 
# FYE107-10VL (10 preps)
Efficiency >1 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE107-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE108-10VL (10 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE108-80VL (80 preps)

Efficiency >5 x 108 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE109-10VL (10 preps)

Efficiency >2 x 109 cfu/μg
100 μl/vial 10 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE109-80VL (80 preps)

Efficiency >2 x 109 cfu/μg
100 μl/vial 80 vials
Control Plasmid (pUC19) 5 μl (10-4 μg/μl)

 

# FYE107-96WL (96 preps)

Efficiency >1 x 108 cfu/μg
50 μl/well 96-wells

 

# FYE108-96WL (96 preps)

Efficiency >5 x 108 cfu/μg
50 μl/well 96-wells

 

# FYE109-96WL (96 preps)

Efficiency >1 x 109 cfu/μg
50 μl/well 96-wells
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