EGF, rat recombinant | 4024

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.

4024 | EGF rat recombinant DataSheet

Biomolecule/Target: EGF

Synonyms: Urogastrone, URG, EGF, epidermal growth factor

Alternates names: Interferon 2b, IFNA, INFA2, MGC125764, MGC125765

Taglines: A growth factor stimulating cell growth, proliferation, and differentiation by binding to EGFR

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2b

Gene Source: Rat

Accession #: Q86UP4

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6 x 10 units/mg

Results: <0.1 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.3 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from PBS, pH 7.4

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A