EGF Receptor Antibody [B1D8] | 33-120

EGF Receptor Antibody [B1D8] | 33-120 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Microsomes from A431 cells were used as the immunogen for this antibody.

Research Area: Cancer, Signal Transduction

Tested Application: Flow, IP, Inhibition

Application: Functional Activity (Inhibition) : antibody blocks EGF receptor activation
inhibits A431 cell proliferation
arrests tumor growth in vivo
affinity purification
IP: 1-2��g/500ug protein lysate
Flow Cytometry: 0.5-1 ug/million cells
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Specificiy: Does not react with mouse and rat

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G purified monoclonal antibody

Clonality: Monoclonal

Clone: B1D8

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa��induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.��EGFR��is type I receptor tyrosine kinase with sequence homology to��ErbB2, -3 -4 or��HER2, -3 -4. It binds to Epidermal Growth Factor (EGF) , ��Transforming Growth Factor-a (TGFa) , Heparin-binding EGF (HB-EGF) , amphiregulin, betacellulin and epiregulin.