EGF Receptor, human recombinant | 7135

EGF Receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including TGF, HBEGF, Betacellulin (BTC), Amphiregulin, Epiregulin, and Epigen. Upon activation, EGFR initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. Recombinant soluble human EGFR is a 621 amino acid glycoprotein comprising the extracellular domain of EGFR, and migrates at an apparent MW of 97.5 kDa by SDS-PAGE analysis under reducing conditions.

7135 | EGF Receptor human recombinant DataSheet

Biomolecule/Target: EGFR

Synonyms: ErbB1

Alternates names: Interferon 2b, IFNA, INFA2, MGC125764, MGC125765

Taglines: Initiates a signaling cascade

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2b

Gene Source: Human

Accession #: Q86UP4

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.6 x 10 units/mg

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.3 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A