EIF2AK1 polyclonal Antibody | BS61712

EIF2AK1 polyclonal Antibody | BS61712 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: HRI (heme-regulated inhibitor kinase) phosphorylates the α subunit of eIF2α kinase, which plays an important role in translational regulation during heme deficiency. HRI is activated in response to a number of environmental conditions, including heme deficiency, heat shock, and oxidative stress. Autophosphorylation is essential for the activation of HRI, which causes an arrest of initiation of protein synthesis. Both HSP 90 and HSC 70 are necessary for all stress-induced HRI activation. Furthermore, HSC 70 is required for the folding and transformation of HRI into an active kinase and is subsequently required to negatively attenuate the activation of transformed HRI. Both the N-terminus and the kinase insertion domain, which are unique to HRI, are involved in the heme binding and the heme regulation of HRI. The human HRI gene maps to chromosome 7p22.1 and encodes a 630 amino acid protein expressed mainly in erythroid cells.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EIF2AK1 polyclonal Antibody detects endogenous levels of EIF2AK1 protein.

Molecular Weight: ~ 71 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase; EIF2AK1; HRI; KIAA1369; PRO1362

Immunogen: Synthetic peptide, corresponding to amino acids 580-630 of Human EIF2AK1.

Conjugate: N/A

Modification: N/A

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: N/A